Version
- Product Details
- Antigen Details
- Documentation
- Reviews
- Related Protocols
- Related Products
- Related FAQs
- Other Formats
- Certificate of Analysis
- Clone
- J252D4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BLR1, MDR15
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Compare all formats See Brilliant Violet 750™ spectral data
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 356941 | 25 tests | £148 | ||||
| 356942 | 100 tests | £298 | ||||
Description
CD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkittâs lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).
Product Details
Technical Data Sheet (pdf)
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human CXCR5-transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 750⢠under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
- FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 750⢠excites at 405 nm and emits at 750 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 750⢠is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violetâ¢.This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
- Violet Laser (405 nm)
- Product Citations
-
- Den Braanker H, et al. 2021. Front Immunol. 12:768113. PubMed
- RRID
- AB_2832703 (BioLegend Cat. No. 356941)
AB_2832704 (BioLegend Cat. No. 356942)
Antigen Details
- Structure
- G-protein coupled receptor, 7 transmembrane regions, 42 kD
- Distribution
-
Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.
- Function
- Mediates cell migration to the B cell follicles in the secondary lymphoid organs
- Ligand/Receptor
- CXCL13
- Cell Type
- B cells, Tfh
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Ma CS, et al. 2012. J. Exp. Med. 209:1241.
2. León B, et al. 2012. Nat. Immunol. 13:681.
3. Crotty S. 2011. Annu. Rev. Immunol. 29:621.
4. Kerfoot SM, et al. 2011. Immunity 34:947.
5. Sáez de Guinoa J, et al. 2011. Blood 118:1560. - Gene ID
- 643 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD185
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD185
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC
- UniProt
- View information about CD185 on UniProt.org
Documentation
- Technical Data Sheet (pdf)
- Certificate of Analysis
- Safety Data Sheet
Reviews
Review This Product
Related Protocols
- Cell Surface Flow Cytometry Staining Protocol
Related Products
| Description | Clone | Applications |
|---|
Pathways
- Chemokine Receptor Biology
- Dendritic Cells
- Immunologic Networks 2011
- T Follicular Helper Cell Pathway
Pages
- Quality Control
- Brilliant Violet™
- Multicolor Staining Guide
- Fluorescence Spectra Analyzer
- CD Molecule Chart
- Fluorophore Brightness Index
- Custom Panel Construction
- Fluorophore Equivalency Tool
Related FAQs
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
-
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
Other Formats
View All CD185 Reagents Request Custom Conjugation
| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD185 (CXCR5) | J252D4 | FC |
| APC anti-human CD185 (CXCR5) | J252D4 | FC |
| Purified anti-human CD185 (CXCR5) | J252D4 | FC |
| Alexa Fluor® 647 anti-human CD185 (CXCR5) | J252D4 | FC |
| PerCP/Cyanine5.5 anti-human CD185 (CXCR5) | J252D4 | FC |
| Alexa Fluor® 488 anti-human CD185 (CXCR5) | J252D4 | FC |
| FITC anti-human CD185 (CXCR5) | J252D4 | FC |
| Alexa Fluor® 700 anti-human CD185 (CXCR5) | J252D4 | FC |
| Pacific Blue™ anti-human CD185 (CXCR5) | J252D4 | FC |
| Brilliant Violet 421™ anti-human CD185 (CXCR5) | J252D4 | FC |
| PE/Cyanine7 anti-human CD185 (CXCR5) | J252D4 | FC |
| APC/Cyanine7 anti-human CD185 (CXCR5) | J252D4 | FC |
| PE/Dazzle™ 594 anti-human CD185 (CXCR5) | J252D4 | FC |
| Brilliant Violet 605™ anti-human CD185 (CXCR5) | J252D4 | FC |
| Biotin anti-human CD185 (CXCR5) | J252D4 | FC |
| Brilliant Violet 711™ anti-human CD185 (CXCR5) | J252D4 | FC |
| Brilliant Violet 785™ anti-human CD185 (CXCR5) | J252D4 | FC |
| TotalSeq™-A0144 anti-human CD185 (CXCR5) | J252D4 | PG |
| TotalSeq™-C0144 anti-human CD185 (CXCR5) | J252D4 | PG |
| Brilliant Violet 750™ anti-human CD185 (CXCR5) | J252D4 | FC |
| TotalSeq™-B0144 anti-human CD185 (CXCR5) | J252D4 | PG |
| APC/Fire™ 750 anti-human CD185 (CXCR5) | J252D4 | FC |
| Spark NIR™ 685 anti-human CD185 (CXCR5) | J252D4 | FC |
| TotalSeq™-D0144 anti-human CD185 (CXCR5) | J252D4 | PG |
| PE/Cyanine5 anti-human CD185 (CXCR5) | J252D4 | FC |
| PE/Fire™ 700 anti-human CD185 (CXCR5) Antibody | J252D4 | FC |
| APC/Fire™ 810 anti-human CD185 (CXCR5) Antibody | J252D4 | FC |
| PerCP/Fire™ 806 anti-human CD185 (CXCR5) | J252D4 | FC |
| PerCP/Fire™ 780 anti-human CD185 (CXCR5) | J252D4 | FC |
Certificate of Analysis
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For Research Use Only. Not for diagnostic or therapeutic use.
This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for userâs intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.
BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.
8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
PE anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
APC anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
Purified anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 647 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
PerCP/Cyanine5.5 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 AP... -
Alexa Fluor® 488 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 AP... -
FITC anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 AP... -
Alexa Fluor® 700 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
Pacific Blue™ anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
Brilliant Violet 421™ anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 Al... -
PE/Cyanine7 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
APC/Cyanine7 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
PE/Dazzle™ 594 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
Brilliant Violet 605™ anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 AP... -
Biotin anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 AP... -
Brilliant Violet 711™ anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 AP... -
Brilliant Violet 785™ anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 AP... -
TotalSeq™-A0144 anti-human CD185 (CXCR5)
-
TotalSeq™-C0144 anti-human CD185 (CXCR5)
-
Brilliant Violet 750™ anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
TotalSeq™-B0144 anti-human CD185 (CXCR5)
-
APC/Fire™ 750 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes stained with CD19 Pacific... -
Spark NIR™ 685 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes stained with CD19 PE and ... -
TotalSeq™-D0144 anti-human CD185 (CXCR5)
-
PE/Cyanine5 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 700 anti-human CD185 (CXCR5) Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Fire™ 810 anti-human CD185 (CXCR5) Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Fire™ 806 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Fire™ 780 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with anti-hu...
